Inverse correlation between the expression of GPR55 and miR-675-5p in clinical specimens of NSCLC. (A, B, C) The expression of GPR55 was assessed in normal and NSCLC tissues using immunohistochemistry. The images were representative IHC of GPR55 in normal, squamous carcinoma, and adenocarcinoma, respectively. (D) A scatter diagram showed an inverse correlation between the expression of miR-675-5p and GPR55 in the same set of NSCLC tissue specimens. (original magnification 400).
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Six NSCLC cell lines (HTB-182, A549, SPC-A-1, H1299, 95-D, Ltep-a-2) were obtained from the American Type Culture Collection. A normal human bronchial epithelial cell line (HBE), were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). Cells were cultured in RPMI 1640 (GIBCO-BRL) medium supplemented with 10% fetal bovine serum (10% FBS), 100 U/ml penicillin, and 100 μg/ml streptomycin (Biyuntian, China) in humidified air at 37C with 5% CO2. 2ff7e9595c
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